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Submission guideline

Requirements will vary depending on the type of GeneChip® you are interested in. Please contact us for detailed sample submission guidelines.

For Platinum service we require enough amount of sample to perform the extraction.  For DNA analysis, whole genome amplification of genomic DNA is possible if sufficient sample is not available.

For Gold service we will require enough quantity of DNA/RNA to perform the GeneChip® processing plus additional material to perform a quality check.

It is recommended that you send as much RNA/DNA as possible as this will allow us to repeat any failed samples immediately without your having to obtain new samples or do the extraction again. This is particularly important if you are shipping internationally as losses may occur in transit.  Any samples that fail quality control will NOT be used unless you specifically ask us to proceed with analysis.

DNA analysis

Gold Service: DNA minimum 500ηg at 50ηg/µl in reduced EDTA TE buffer (0.1mM EDTA, 10mM Tris, pH 8.0).
(At least 2µg is recommended to allow all QC checks and repeat reactions
if necessary.)

Silver service: At least 230µg of labeled DNA prepared according to the Affymetrix protocols.

Expression analysis

Gold Service: Total RNA minimum 5µg at 1µg/µl in DEPC-treated water. (At least 15-20µg of Total RNA is recommended to allow all QC and repeat reactions if necessary.)
Silver Service: At least 45µg of purified biotinylated cRNA prepared according to the Affymetrix Expression Manual Protocols.

 

Information

To learn more about the Affymetrix GeneChip®, including complete product protocols and tutorials, visit affymetrix.com.

For a complete list of all the GeneChips® available see -
Affymetrix GeneChip® system

The CustomExpress program allows you to design arrays to your specification for your research. More information in pdf format

Affymetrix has recently launched its Cytogenetics Analysis solution using SNP 6.0 GeneChips®

 

 

 

     
   
     
   
     
 
 
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