IEF:

VIMTA is endowed with the new-generation tandem time-of-flight MS/MS system - ABI4800 MALDI TOF/TOF™ Analyzer

MS-based Protein Identification -

both peptide mass fingerprinting (PMF) and MS/MS peptide fragmentation

Peptide Mass Fingerprint (PMF) -

protein sample is digested using different proteases. These protein fragments are accurately measured using MALDI-TOF spectrometry to produce a “fingerprint”, unique to each protein. The protein is then identified from the MASCOT protein database.

MS/MS peptide fragmentation -

Automated spot Picker is used for the precise excision of protein of interest for further proteomic analysis

MS/MS peptide fragmentation -