To order sequencing services you can register online and fill in the service request form. Alternatively, our sample order form can be downloaded from the website, and the completed form sent to us along with the samples. Please send the samples to :
Life Sciences Campus
#5, S.P.Biotech Park, Genome Valley
Shameerpet, Hyderabad - 500 078
Andhra Pradesh, India
Tel : +91 40 3984 8484
Email: vimtahq@vimta.com
If you are sending large quantities of samples from within Hyderabad, you can use our free courier service. Call us at 91-40-39848484 Ext: 4302 for further details. No sequencing reactions will be performed until Vimta has the completed order information including the purchasing information.
For each sequencing reaction, customers should send 1 µg of template DNA and at least 10 pmoles of primer in a final volume of 10 µl. If you are sequencing with a universal primer or if Vimta has your sequencing primer, the tube should only contain 1 µg of template in a total volume of 9 µl. We will add 1 µl (containing 10 pmol) of primer.
A control sequencing reaction is performed with each set of customer samples. The template is PGEM with M13 primer. The quality of the control sequencing chromatogram (available upon request at no cost) is used to evaluate the quality of the overall reactions. If a sequencing reaction is of poor quality, but the quality of the control sequence is good, the reactions will be billed.
Once the sequencing reactions are complete, hard copies of the sequence chromatograms, text versions of the sequence, and/or an electronic text copy of the sequence can be provided.
Currently, we are not preparing sequencing templates. If you choose to prepare your own template, we recommend using a commercially available kit such as Qiagen MINI-Prep or Promega Wizard mini-prep system. The purity of the DNA template directly influences the quality of your DNA sequences.
